- Ready-to-use, sterile, endotoxin-tested solution
- Highly effective for the purification of human mononuclear cells (monocytes and lymphocytes) from diluted blood
Please note The cat. The LymphoPrep™ (1x250ml) number changes from 1114544 to 1856-1 but the product itself has not changed in any way.
Intended Use: For Research Only
Lymphoprep™ is a ready-to-use, sterile, endotoxin-tested solution for the isolation of suspensions of pure lymphocytes. The solution contains sodium diatrizoate and polysaccharides.
Density: 1.077 ± 0.001 g/ml
Composition: 9.1% (w/v) sodium diatrizoate, 5.7% (w/v) polysaccharide
Osmolality: 290 ± 15 mOsm
Endotoxin: < 1.0 EU/ml
Stability and storage
Lymphoprep™ is stable for 3 years as long as the solution is kept sterile and protected from light. Prolonged exposure to direct sunlight causes the release of iodine from the sodium diatrizoate molecule. This effect is negligible when working with this solution on a day-to-day basis. Lymphoprep™ should be stored between +4°C and +30°C.
In 1968, Dr Arne Boyum reported a simple and effective method for the isolation of mononuclear cells from human blood. For more than 35 years, a commercial medium known as Lymphoprep has been widely used to isolate these cells.
Mononuclear cells (monocytes and lymphocytes) have a lower buoyant density than erythrocytes and polymorphonuclear (PMN) leukocytes (granulocytes). The vast majority of mononuclear cells have densities less than 1.077 g/ml. Therefore, these cells can be isolated by centrifugation in an isoosmotic medium with a density of 1.077 µg/mL, allowing erythrocytes and PMNs to sediment in the medium while retaining mononuclear cells at the sample/medium interface.
The described method is fast, simple and reliable and gives excellent results with blood samples from normal individuals and patients. For maximum performance, it is important that the blood sample is diluted 1:1 with normal saline before applying it to the gradient. Contamination of erythrocytes in the mononuclear cell suspension is usually between 3-10% of the total number of cells.
Some immature PMNs can bind to lymphocytes during intense immunosuppressive therapy. When heparinized blood is used, it is essential to remove most of the platelets, to avoid inhibition in the cytotoxicity assay. LymphoprepTM can be used for the preparation of pure lymphocyte suspensions for tissue typing, antilymphocyte sera, and immunological research. Thorsby and Brattelie used this technique with slight modifications in the preparation of pure lymphocyte suspensions for cytotoxicity tests and lymphocyte cultures.