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Wang et al. Bead capture increases the sensitivity of sputum microscopy for the diagnosis of tuberculosis in Beijing, China. Trans Royal Soc Trop Med Hyg. 2013;
ABSTRACT. Methods; A total of 129 sputum samples were investigated by direct smear microscopy, microscopy after TB-Bead extraction and by solid and liquid culture. Results; Direct smear had a sensitivity of 40% by Ziehl-Neelsen (ZN) and 45% by auramine compared to combined culture results. After TB-Bead extraction, this increased to 65% for ZN and 70% for auramine. Conclusion; Magnetic bead concentration of mycobacteria from sputum led to a significant improvement in the sensitivity of microscopy compared with direct smear.
Liu J, Sun Z-Q,Peia H et al. Increased case finding of tuberculosis from sputum and sputum deposits after magnetic bead concentration of mycobacteria. J Microbiol Methods; 2013; 93(2):144-7.
78 sputa were tested as a blind panel by TB-Beads in combination with traditional direct smear. Direct smear had a sensitivity of 33.3% whereas TB-Beads had a sensitivity of 46.2%. In this study, TB-Beads enabled case detection to be increased by 38.7% compared to direct smear and enabled 10 more patients to be detected from a study population of 78 patients. 29 sputum deposits that were negative by initial smear were re-examined by microscopy after extraction by TB-Beads. In total 4/29 of previously negative sputum deposits were found to be microscopy positive after concentration by TB-Bead extraction. This is an increase in case finding of 14%. Concentration of mycobacteria from the sputum deposits by TB-Beads increased the numbers of positive samples identified by microscopy.
Ghodbane R, Drancourt M. Magnetic bead protocol for culturing Mycobacterium tuberculosis from Sputum samples. J Clin Microbiol 2013; 51(5): 1578. 2013.
ABSTRACT. A magnetic bead protocol and a standard centrifugation protocol yielded Mycobacterium tuberculosis in 40/50 sputum specimens in 12 ± 1 days and 11 ± 2 days, respectively. Manipulation took 35 ± 5 min and 45± 10 min, respectively. The magnetic bead protocol could advantageously replace centrifugation for culturing M. tuberculosis from sputum.
Ohkuma M, Ikeda K, Obayashi K, et al. Evaluation of TB-beads assay utilizing the technique of magnetic beads- an innovative assay method for detection of acid fast bacilli. Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi. 2012;23(1):1-9.
QUOTE. ‘TB-beads is an easy method that allows to simplify the process of smear tests, and contributes to significantly reducing the contamination rate of culture tests. It also contributes to improving the sensitivity and detection rate of AFB testing. Furthermore, it does not require centrifugation. Ultimately, TB-beads is an innovative, safe, and convenient testing method for detection of AFB, which enables laboratory technicians to save time for routine work.’
Wilson S, Lane A, Rosedale R, Stanley, C. 2010. Concentration of Mycobacterium tuberculosis from sputum using ligand-coated magnetic beads. Int J Tuber Lung Dis 2010; 14 (9): 1164-1168.